AmoyDx® Super-ARMS ESR1 PCR Kit
PCR
Inquiry
The AmoyDx® Super-ARMS ESR1 PCR Kit is a qualitative real-time PCR assay designed to detect 29 somatic mutations in the ESR1 gene across exons 4, 5, 6, 7, and 8 using circulating free DNA (cfDNA) extracted from plasma samples. cfDNA, derived from apoptotic and necrotic tumor cells, is detectable in the peripheral blood of breast cancer patients. Plasma-based detection of ESR1 mutations provides a non-invasive method for assessing mutation status, particularly in late-stage breast cancer cases, as cfDNA in early-stage patients is often more fragmented and present at lower concentrations.
Comprehensive ESR1 Gene Coverage
Covers 29 ESR1 mutations
Limit of detection
as low as 0.2%
Cost-efficient
Turnaround time
within 5.5 hours
Specifications
Tests/kit
14
Instrument
QuantStudio5, SLAN-96S
Sample type
cfDNA extracted from plasma sample
Technology
With Super-ARMS, efficient amplification occurs when the 3’ base of the primer perfectly matches the mutant template. Conversely, if the 3’ base mismatches with the wild-type template, amplification efficiency is significantly reduced.
The refined primer design significantly enhances both specificity and sensitivity, offering improved performance in mutation detection.
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