BRAF Mutation Detection Kit(V2)
For Research Use Only
PCR
Real-time PCR Assays
Product Description

BRAF is a serine/threonine kinase that functions within the Ras-Raf-MEK-MAPK pathway. This pathway normally regulates cell proliferation and survival under the control of growth factors and hormones. Mutations in the BRAF gene have been associated with the development of cancer. The most common alteration in the BRAF gene is a mutation called V600E, which alters the valine at position 600 in the protein to a glutamic acid. Other mutations observed at the V600 position are V600D, V600K, V600R and V600A. BRAF mutations occur in ~50% of melanoma, ~40% of papillary thyroid tumors, 1~4% of non-small cell lung cancer (NSCLC), ~30% of ovarian tumors, ~10% of colorectal tumors, ~10% of prostate tumors. In the clinical studies, BRAF targeted therapy is associated with clinical benefit in majority of patients with BRAF V600 mutations. BRAF inhibitors and MEK inhibitors have been approved for treatment of patients with BRAF V600-mutated melanoma, NSCLC and thyroid cancer.
Intended Use
The AmoyDx® BRAF Mutation Detection Kit (V2) is a real-time PCR assay for qualitative detection of V600E, V600E2, V600K, V600D, V600D2, V600A, V600R mutations in BRAF gene in human genomic DNA extracted from tumor tissue sample.
The kit is for research use only, and intended to be used by trained professionals in a laboratory environment.

 

Download
Testing Procedure
Compatible Sample Types:FFPE tissue
Compatible Real-time PCR Instrument
SLAN-96S
Rotor-Gene Q
LightCycler480
Bio-Rad CFX96
Certificate
BACK
Inquiry
Inquiry
* Required information
Manage your account info
Change your password