KRAS/NRAS Mutations Detection Kit
PCR
Real-time PCR Assays
100%
100%
Specificity
1%
1%
Sensitivity
Features
Selectable
- Pre-loaded and bulk formats are available.
1
- Pre-loaded and bulk formats are available.
Universal
- Compatible with tissue samples.
2
- Compatible with tissue samples.
Precise
- Top sensitivity of 1% mutant allele.
3
- Top sensitivity of 1% mutant allele.
Easy
- One-step procedure, results in 90 mins.
4
- One-step procedure, results in 90 mins.
Reliable
- Internal and external controls included.
5
- Internal and external controls included.
Product Description

KRAS/NRAS Mutations Detection Kit

RAS protein is a GTPase and one of the key molecules in the downstream signaling pathway of epidermal growth factor receptor (EGFR). RAS protein transduces signals from membrane-bound receptors via multiple downstream effector pathways and thereby affects fundamental cellular processes, including proliferation, apoptosis, and differentiation. In total, activating KRAS and NRAS mutations occur in 20~50% and 1~6% of colorectal cancer respectively, mainly in exons 2, 3 or 4. The mutation status of the RAS gene is relevant to the primary drug resistance of colorectal cancers treated with anti-EGFR monoclonal antibodies. Patients with wild-type KRAS and NRAS gene could benefit from Erbitux (Cetuximab) or Vectibix (Panitumumab), whereas, the patients with mutant KRAS or NRAS gene show poor response to this treatment. RAS testing is recommended for the selection of colorectal cancer patients to receive anti-EGFR antibody therapy by NCCN Clinical Practice Guideline in Oncology and European Drug Administration Organization.

Intended Use

The AmoyDx® KRAS/NRAS Mutations Detection Kit is a real-time PCR assay for qualitative detection of 19 KRAS mutations (exons 2, 3 and 4)and 13 NRAS mutations (exons 2, 3 and 4) in human genomic DNA extracted from formalin-fixed paraffin-embedded (FFPE) tumor tissue. The kit is intended to assess KRAS/NRAS mutation status in colorectal cancer patients.

The kit is for in vitro diagnostic use, and intended to be used by trained professionals in laboratory environment.

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Testing Procedure
Compatible Sample Types:FFPE Samples
Compatible Real-time PCR Instrument
ABI 7500
Bio-Rad CFX96
LightCycler 480
SLAN-96S
Stratagene Mx3000P™
Other Info.

To order, please contact sales@amoydx.com.

 Product Name

Type

Size (test/kit)

 Storage

KRAS/NRAS Mutations Detection Kit

Pre-loaded

6

-20℃

KRAS/NRAS Mutations Detection Kit

 Bulk

 24

-20℃

 

Publications
1. Kamarádová, Kateřina, et al. "Non-conventional mucosal lesions (serrated epithelial change, villous hypermucinous change) are frequent in patients with inflammatory bowel disease—results of molecular and immunohistochemical single institutional study." Virchows Archiv (2019): 1-11.

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