KRAS Mutation Detection Kit
PCR
Real-time PCR Assays
19
Mutations
1%~5%
1%~5%
Sensitivity
Features
Selectable
- Pre-loaded and bulk formats are available.
1
- Pre-loaded and bulk formats are available.
Universal
- Compatible with FFPE tissue samples.
2
- Compatible with FFPE tissue samples.
Precise
- Top sensitivity of 1% mutant allele.
3
- Top sensitivity of 1% mutant allele.
Easy
- One-step procedure, results in 90 mins.
4
- One-step procedure, results in 90 mins.
Reliable
- Internal and external controls included.
5
- Internal and external controls included.
Product Description

KRAS Mutation Detection Kit
KRAS protein is a GTPase and one of the key molecules in the downstream signaling pathway of epidermal growth factor receptor (EGFR). KRAS protein transduces signals from membrane-bound receptors via multiple downstream effector pathways and thereby affects fundamental cellular processes, including proliferation, apoptosis, and differentiation. In total, activating mutations in the KRAS genes occur in 15~30% of lung cancer and 20~50% of colorectal cancer, mainly in exons 2, 3 or 4. The mutation status of the KRAS gene is relevant to the primary drug resistance of colorectal cancers treated with anti-EGFR monoclonal antibodies. Patients with wild-type KRAS gene could benefit from Erbitux (Cetuximab) or Vectibix (Panitumumab), whereas, the patients with mutant KRAS gene show poor response to this treatment. The European Drug Administration Organization and US FDA approve the employment of KRAS gene mutation detection prior to the use of targeted medicines in the treatment of metastatic colorectal cancer.
Intented Use

The AmoyDx® KRAS Mutation Detection Kit is a real-time PCR assay for qualitative detection of 19 somatic mutations in codons 12, 13, 59, 61, 117 and 146 of KRAS gene in human genomic DNA extracted from formalin-fixed paraffin-embedded (FFPE) tumor tissue. The kit is intended to assess KRAS mutation status in colorectal cancer patients.

The kit is for in vitro diagnostic use, and intended to be used by trained professionals in a laboratory environment.

Testing Procedure
Compatible Sample Types:FFPE Samples
Compatible Real-time PCR Instrument
ABI7500
cobas z480
Bio-Rad CFX96
LightCycler480
SLAN-96S
Stratagene Mx3000P™
Other Info.

To order, please contact sales@amoydx.com.

 Product Name

Type

 Size (test/kit)

Strorage

KRAS Mutation Detection Kit

Pre-loaded

6T

-20±5 ℃

KRAS Mutation Detection Kit

Bulk

24T

-20±5 ℃


Publications
1. Ye, Ju-Xiang, et al. "KRAS and BRAF gene mutations and DNA mismatch repair status in Chinese colorectal carcinoma patients." World journal of gastroenterology: WJG 21.5 (2015): 1595.
2. Hagan, Suzanne, Maria CM Orr, and Brendan Doyle. "Targeted therapies in colorectal cancer—an integrative view by PPPM." EPMA Journal 4.1 (2013): 3.
3. Trachu, N., et al. "Molecular alterations and clinical prognostic factors for cholangiocarcinoma in Thai population." OncoTargets and therapy 10 (2017): 4955.

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