EML4-ALK Fusion Gene Detection Kit
PCR
Ensayos de PCR en tiempo real
100%
100%
Specificity
100%
100%
Accuracy
25 copies/μL
Sensitivity
Features
Selectable
- Pre-loaded and bulk formats are available.
1
- Pre-loaded and bulk formats are available.
Universal
- Compatible with tissue samples.
2
- Compatible with tissue samples.
Precise
- Top sensitivity of 125 copies/rxn.
3
- Top sensitivity of 125 copies/rxn.
Easy
- Two steps procedure, results in 150 mins.
4
- Two steps procedure, results in 150 mins.
Reliable
- External control included.
5
- External control included.
Product Description

EML4-ALK Fusion Gene Detection Kit

The AmoyDx EML4-ALK Fusion Gene Detection Kit is a sensitive and selective assay for the detection of the twenty-one most common fusion transcripts of the chimeric EML4-ALK gene.

The EML4-ALK fusion oncogene represents one of the newest molecular targets in non-small-cell lung cancer (NSCLC). First described in 2007, the fusion results from a small inversion within chromosome 2p. This inversion leads to expression of a chimeric tyrosine kinase having the N-terminal half of echinoderm microtubule-associated protein-like 4 (EML4) fused to the intracellular kinase domain of anaplastic lymphoma kinase (ALK). The EML4-ALK protein possesses potent oncogenic activity both in vitro and in vivo. This activity can be blocked by small-molecule inhibitors, supporting a role for EML4-ALK as a key driver of lung tumorigenesis.

Technological Principles:

The EML4-ALK kit employs proprietary real-time PCR technology to detect the twenty-one most common EML4-ALK fusion transcripts. RNA that has been purified from the FFPE tissue is first reverse transcribed to cDNA with RT enzyme supplied with the kit. The EML4-ALK fusion cDNAs are then specifically amplified with cross-junction PCR primers and quantitatively detected with fluorescent probes.

Testing Procedure
Compatible Sample Types:FFPE Samples
Compatible Real-time PCR Instrument
ABI 7500
Bio-Rad CFX96
LightCycler 480
Rotor-Gene Q
Rotor-Gene 6000
SLAN-96S
Stratagene Mx3000P™
Other Info.

To order, please contact sales@amoydx.com.

AmoyDx EML4-ALK Fusion Gene Detection Kits are available in the following size, minimum order of 12 tests:

 Product Name

Size (tests/kit) 

Storage 

 Cat. No.

 EML4-ALK Fusion Gene Detection Kit (Bulk)

 24T

 -20 

8.01.22001X024H

  EML4-ALK Fusion Gene Detection Kit (Pre-loaded)

 12T

 -20 

8.01.22001W012A
8.01.22001W012B
8.01.22001W012D
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